Research Paper Volume 12, Issue 14 pp 14300—14313

Caveolin-3 protects diabetic hearts from acute myocardial infarction/reperfusion injury through β2AR, cAMP/PKA, and BDNF/TrkB signaling pathways

Figure 3. An ADRB2 agonist activates cAMP/PKA signaling and the recovery of H9C2 cells from H/R injury under high-glucose (HG) stimulation. H9C2 cells were subjected to H/R injury with or without HG stimulation and ADRB2 agonist ISO treatment and examined for (A, B) the cell apoptosis by Flow cytometry (n=3); (C, D) the protein levels of apoptotic Bax/Bcl-2 signaling factors Bax and Bcl-2 by immunoblotting (n=3); (E) the cell viability by MTT assay (n=5); (F) the cAMP concentrations (n=3); (G, H) the protein levels of p-PKA, PKA, TrkB, p-Akt, and Akt by immunoblotting, (n=3).**P<0.01, compared to control group; #P<0.05, ##P<0.01, compared to H/R group; &&P<0.01, compared to H/R + HG group.