Research Paper Volume 12, Issue 14 pp 14300—14313

Caveolin-3 protects diabetic hearts from acute myocardial infarction/reperfusion injury through β2AR, cAMP/PKA, and BDNF/TrkB signaling pathways

Figure 5. Caveolin-3 enhances the activity of BDNF/TrkB signaling through ADRB2 and cAMP. (A, B) ADRB2 silencing was conducted in H9C2 cells by transfection of si-ADRB2, as confirmed by immunoblotting. Next, under H/R+HG stimulation, H9C2 cells were co-transfected with pcDNA3.1/Caveolin-3 and si-ADRB2 and examined for (C, D) cAMP, p-PKA, PKA, TrkB, p-Akt, and Akt proteins. N=3. **P<0.01 compared to si-NC group or pcDNA3.1+si-NC group; ##P<0.01, compared to pcDNA3.1+si-ADRB2 group.