Research Paper Volume 12, Issue 14 pp 14314—14328

Circular RNA-9119 suppresses in ovarian cancer cell viability via targeting the microRNA-21-5p–PTEN–Akt pathway

Figure 3. circ9119 induced apoptosis in ES-2 and SKOV-3 cells. Cells were subjected to transfection with circ9119 overexpression or NC vector for 1.5 d. (A, B) Flow cytometry results displayed the number of apoptotic cells. (C, D) Caspases activity detection kit was used to detect the activity of caspase 3, caspase 8, and caspase 9 post transfection. (E, F) Western blotting was conducted to examine the expression of caspase 3, caspase 8, and caspase 9 and their cleavage forms. Actin represents β-actin. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the NC groups.