Research Paper Volume 12, Issue 14 pp 14314—14328

Circular RNA-9119 suppresses in ovarian cancer cell viability via targeting the microRNA-21-5p–PTEN–Akt pathway

Figure 4. circ9119 targets miR-21. (A) Bioinformatics forecasting showed an miR-21 binding site in the circ9119 RNA sequence. (B) DLRA was conducted and a luciferase reporter containing WT or mutant circ9119 and the miR-21 mimic were subsequently cotransfected into HEK293T cells. (C) qRT–PCR displayed the miR-21 expression in HCC samples (n = 40) and paired adjacent normal healthy control samples (n = 10). (D) qRT–PCR displayed miR-21 expression in FTE187 and OC cell lines. (E, F) ES-2 and SKOV-3 cells were transfected with circ9119 overexpressing or NC vector. qRT–PCR examined miR-21 expression. n = 3. *P < 0.05, **P < 0.01 vs. specific groups.