Research Paper Volume 12, Issue 14 pp 14314—14328

Circular RNA-9119 suppresses in ovarian cancer cell viability via targeting the microRNA-21-5p–PTEN–Akt pathway

Figure 5. miR-21 mimic reversed the role of circ9119 overexpression on viability and apoptosis of ES-2 and SKOV-3. Cells were subjected to cotransfection with circ9119 overexpressing and miR-21 or NC mimic for 1.5 d. (A, B) qRT–PCR was examined the miR-21 expression. (C, D) MTT was conducted to detect the proliferation rate of SKOV-3 and ES-2 cells under different transfection conditions. (E, F) Colony formation assay indicated the growth of ES-2 and SKOV-3 cells under various transfection conditions. (G, H) Caspase activity detection kit was used to detect the activity of caspase 3, caspase 8, and caspase 9 after transfection. (I, J) Western blotting was conducted to examine the caspase-3, -8, and -9 expression and their cleavage form. Actin represents β-actin. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001 vs. specific groups.