Research Paper Volume 12, Issue 14 pp 14314—14328

Circular RNA-9119 suppresses in ovarian cancer cell viability via targeting the microRNA-21-5p–PTEN–Akt pathway

Figure 6. miR-21 targets the 3′-UTR of PTEN mRNA. (A) Bioinformatics forecasting displayed that miR-21 possesses a binding site in PTEN 3’-UTR. (B) DLRA was conducted and a luciferase reporter containing WT or MU of PTEN mRNA and miR-21/NC mimic were cotransfected into HEK293T cells. (C) qRT–PCR showed PTEN mRNA expression levels in patients with OC (n = 40) and healthy controls (n = 10). (D) qRT–PCR showed the expression levels of PTEN in FTE187 and OC cell lines. (E, F) ES-2 and SKOV-3 cells were transfected with an miR-21 or NC inhibitor. qRT–PCR and Western blotting were performed to examine PTEN levels. (G, H) SKOV-3 and ES-2 were subjected to cotransfection with circ9119 overexpressing vector and miR-21 or NC mimic. qRT–PCR and western blotting was performed to examine PTEN expression. Actin represents β-actin. n = 3. *P < 0.05 vs. specific groups.