Figure 7. OB treatment inhibited the growth of SKOV-3 and ES-2 cells. Cells were incubated with 5 μmol/L of OB or 1% of DMSO for 12 h. (A, B) Western blotting was used to detect PTEN and Akt expressions as well as the phosphorylated Akt level. Actin represents β-actin. (C, D) Indirect immunofluorescence assay was conducted to observe the localization of Akt. (E, F) Colony formation assay indicated the growth of ES-2 and SKOV-3 cells with OB treatment. n = 3. *P < 0.05 vs. specific groups.