Research Paper Volume 12, Issue 14 pp 14329—14340

Downregulation of hsa_circ_0007580 inhibits non-small cell lung cancer tumorigenesis by reducing miR-545-3p sponging

Figure 4. Hsa_circ_0007580 shRNA2 notably induced the apoptosis and inhibited the invasion of NSCLC cells. (AD) The proportion of apoptotic cells was detected using fluorescence-activated cell sorting after double staining with Annexin V and PI. X axis: the level of Annexin-V fluorescein isothiocyanate fluorescence; Y axis: the PI fluorescence. (EH) The invasion of NSCLC cells was tested with a Transwell invasion assay; 400× magnification. **P<0.01 vs. control.