Figure 4. Hsa_circ_0007580 shRNA2 notably induced the apoptosis and inhibited the invasion of NSCLC cells. (A–D) The proportion of apoptotic cells was detected using fluorescence-activated cell sorting after double staining with Annexin V and PI. X axis: the level of Annexin-V fluorescein isothiocyanate fluorescence; Y axis: the PI fluorescence. (E–H) The invasion of NSCLC cells was tested with a Transwell invasion assay; 400× magnification. **P<0.01 vs. control.