Figure 5. MiR-545-3p was the downstream target of hsa_circ_0007580. (A, B) The gene structure of hsa_circ_0007580 at positions 518-524 contained the predicted target site of miR-545-3p, with a sequence of CCUUAC. (C) The expression of miR-545-3p in A549 cells was detected using qRT-PCR. (D) Luciferase activity was measured with a dual luciferase reporter assay in A549 cells co-transfected with the WT/MT hsa_circ_0007580 plasmid and miR-545-3p. (E) The colocalization of hsa_circ_0007580 and miR-545-3p was detected using FISH. (F) RNA pull-down assay was performed to confirm the correlation between hsa_circ_0007580 and miR-545-3p. **P<0.01 vs. control.