Research Paper Volume 12, Issue 14 pp 14329—14340

Downregulation of hsa_circ_0007580 inhibits non-small cell lung cancer tumorigenesis by reducing miR-545-3p sponging

Figure 8. Hsa_circ_0007580 shRNA2 significantly attenuated the symptoms of NSCLC in vivo. Mice were injected with the vector-control or hsa_circ_0007580 shRNA2. (A) The tumor volumes of the mice were measured weekly for four weeks. (B) At the end of the study, the tumor tissues were collected and photographed. (C) The tumor weights in each group of mice were calculated. (D) The protein levels of PRKCA, p38 and p-p38 in tumor tissues from the mice were detected through Western blotting. (E) The relative expression of PRKCA was quantified and normalized to that of β-actin. (F) The relative expression of p-p38 was quantified and normalized to that of β-actin. **P<0.01 vs. control.