Research Paper Volume 12, Issue 14 pp 14355—14364

LncRNA FGD5-AS1 promotes tumor growth by regulating MCL1 via sponging miR-153-3p in oral cancer

Figure 3. FGD5-AS1 regulates miR-153-3p expression. (A) The paired bases of FGD5-AS1 with miR-153-3p. (B) WT and mutant miR-153-3p luciferase plasmids were transfected into HEK293 cells with pcDNA3.1 or FGD5-AS1. The luciferase activity was measured by dual-luciferase reporter assay system. (*p<0.05). (C) FGD5-AS1 or si- FGD5-AS1 or its NC was transfected into SCC4 cells. The mRNA level of miR-153-3p was analyzed by qRT-PCR (*p<0.05). The mRNA level of miR-153-3p in OC tissues (D) and cells (E) was detected by qRT-PCR (*p<0.05).