Research Paper Volume 12, Issue 14 pp 14355—14364

LncRNA FGD5-AS1 promotes tumor growth by regulating MCL1 via sponging miR-153-3p in oral cancer

Figure 5. MCL1 is direct targets of miR-153-3p. (A) Targeting prediction results of miR-153-3p with MCL1. (B) WT and mutant MCL1 luciferase plasmids were transfected into HEK293 cells with NC or miR-153-3p. The luciferase activity was measured by dual-luciferase reporter assay system. (*p<0.05). miR-153-3p or AMO-miR-153-3p or its NC was transfected into SCC4 cells. (C) The mRNA level of MCL1 was analyzed by qRT-PCR (*p<0.05). (D) Western blot was performed to detect MCL1 protein expression of (*p<0.05). The protein level of MCL1 in OC tissues (E) and cells (F) was detected by western blot (*p<0.05).