Research Paper Volume 12, Issue 14 pp 14365—14375

Circular RNA circVEGFC accelerates high glucose-induced vascular endothelial cells apoptosis through miR-338-3p/HIF-1α/VEGFA axis

Figure 2. circVEGFC knockdown alleviated the apoptosis and proliferation inhibition induced by HG. (A) RT-qPCR showed the level of circVEGFC in the HUVEC cells treated with HG concentration gradient. (B) The expression level of circVEGFC with different time. (C) Short hairpin RNA (shRNA) was synthesized to knock down the circVEGFC expression. (D) CCK-8 assay indicated the proliferative ability of HUVECs with normal glucose (NG)/HG administration or circVEGFC knockdown transfection. (E) Flow cytometry apoptosis unveiled the apoptosis of HUVECs with NG/HG administration or circVEGFC knockdown. (F) Ethynyl-2-deoxyuridine (EdU) demonstrated the proliferation of HUVECs transfected with circVEGFC knockdown or control. *P < 0.05, **P < 0.01.