Figure 4. circVEGFC/miR-338-3p targeted HIF-1α. (A) Bioinformatics tools demonstrated the potential target for circVEGFC/miR-338-3p regulation. (B) Wild type and mutant sequence vectors that containing miR-338-3p binding sites were constructed for luciferase reporter assay. (C) Luciferase reporter assay illustrated the luciferase activity in the co-transfection of miR-338-3p and HIF-1α wild type or mutant. (D) RT-PCR showed the HIF-1α mRNA level in the HG-induced HUVECs. (E) RT-PCR showed the HIF-1α mRNA expression in HUVECs transfected with circVEGFC knockdown or miR-338-3p inhibitor. *P < 0.05, **P < 0.01.