Research Paper Volume 12, Issue 14 pp 14376—14390

MicroRNA-15a/16/SOX5 axis promotes migration, invasion and inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes

Figure 1. MicroRNA-15a/16 targets the 3’UTR of SOX5. (A) Schematic representation of the putative target site for miR-15a/16 in the 3’UTR of SOX5. The binding sites of miR-15a/16 in SOX5 3’UTR were conserved among species. (B) 293T cells were co-transfected with luciferase reporter containing wild-type (WT), mutant (MUT) 3’UTR of SOX5 and miRNA mimics. Mutations within the seed sequence were marked as bold italic. After transfection for 48h, the luciferase intensity was measured and renilla intensity was used as for normalization. Bars show the mean ± SD of 3 independent experiments.