Research Paper Volume 12, Issue 14 pp 14452—14466

Long noncoding RNA LINC01559 promotes pancreatic cancer progression by acting as a competing endogenous RNA of miR-1343-3p to upregulate RAF1 expression

Figure 4. LINC01559 functioned as a ceRNA for miR-1343-3p. (A) RT-qPCR analysis of miR-1343-3p expression in PC tissues and cell lines (B). (C, D) Pearson correlation analysis was used to investigate the correlation between LINC01559 and miR-1343-3p from our data and TCGA. (E) miR-1343-3p expression in shCtrl or shlinc01559 groups. (F) The expression of miR-1343-3p was detected by PCR after transfecting miR-1343-3p mimics or inhibitors in PC cells. (G) The prediction binding site of miR-1343-3p in LINC01559 3’UTR. (H) Luciferase assay and (I) RIP assay were applied to investigate the direct interaction between LINC01559 and miR-1343-3p. (JL) Functional rescue experiments was performed to verify the effect of miR-1343-3p inhibitor on migration and proliferation in shlinc01559 group. *p < 0.05, **p < 0.01, ***p < 0.001.