Research Paper Volume 12, Issue 14 pp 14480—14489

MiR-135a inhibits non-small cell lung cancer progression by suppressing RAB1B expression and the RAS pathway

Figure 2. MiR-135a negatively regulates RAB1B and suppresses NSCLC cell growth and invasion. (A) Putative miR-135a-binding sites in the RAB1B 3’UTR. The nucleotide sequence illustrated the predicted base-pairing between miR-135a and the RAB1B 3’-UTR. (B) Luciferase reporter assays demonstrated that the reporter activity of HEK-293T cells decreased by over 50% upon cotransfection of the wild-type RAB1B 3′-UTR reporter construct and miR-135a mimics. (C, D) Real-time PCR (C) and western blotting (D) analysis of endogenous RAB1B expression in A549 and H1299 cells after transfection with miR-135a mimic vectors. (E, F) A549 and H1299 cells were transfected with RAB1B-specific siRNAs and subjected to proliferation (E) and invasion (F) assays as described above. Data are expressed as the mean ± sd. * p < 0.05; ** p < 0.01; *** p < 0.001.