Figure 2. Gain of ROR1-AS1 promoted cell proliferation of osteosarcoma cells. (A) The ROR1-AS1 expression in osteosarcoma cells (MG-63, U2OS and SAOS-2) and hFOB1.19 was detected by RT-qPCR. (B) The expression of ROR1-AS1 in MG-63 cell after transfected with pcDNA- ROR1-AS1 was detected by RT-qPCR. (C) ROR1-AS1 overexpression increased cell cycle in the MG-63 cell. (D) The expression of cyclin D1 was detected by RT-qPCR. (E) The expression of PCNA was measured by RT-qPCR. (F) The expression of ki-67 was analyzed by RT-qPCR. (G) ROR1-AS1 overexpression induced cell proliferation in MG-63 cell using CCK-8 assay. *p<0.05, **p<0.01 and ***p<0.001.