Figure 6. miR-504 overexpression partly abrogated ROR1-AS1-induced effects on osteosarcoma cell migration and proliferation. (A) Overexpression of miR-504 inhibited cell cycle in ROR1-AS1-overexpressing MG-63 cell. (B) Cell proliferation was measured by CCK-8 analysis. (C) The expression of cyclin D1 was detected by RT-qPCR. (D) The expression of PCNA was measured by RT-qPCR. (E) The expression of ki-67 was analyzed by RT-qPCR. (F) The relative wound closure was shown. *p<0.05, **p<0.01 and ***p<0.001.