Research Paper Volume 12, Issue 14 pp 14718—14735

Demethoxycurcumin analogue DMC-BH inhibits orthotopic growth of glioma stem cells by targeting JNK/ERK signaling

Figure 2. DMC-BH induces apoptosis in GSCs. (A) Morphological changes associated with apoptotic cell death analyzed by TUNEL staining. Red nuclear staining indicates apoptosis, while blue staining suggests normal nuclei. (B) Apoptosis analyzed using Annexin V-FITC/PI-staining flow cytometry. (C) Western analysis of apoptosis-related proteins caspase-3, PARP, and Bcl-2. (D) Caspase-3, -8 and -9 activity measured by ELISA.