Figure 2. DMC-BH induces apoptosis in GSCs. (E) JC-1 positive cells analyzed by flow cytometry. (F) Intracellular ROS generation induced by 100 μM DMC-BH analyzed using DCFH-DA (10 μM) and flow cytometry. (G) Apoptosis of cells treated with DMC-BH and/or z-VAD-fmk for 24 h, analyzed by flow cytometry. (H) Caspase-3 activity measured using the substrate peptide Ac-DEVD-pNA; n =3.