Figure 5. DMC-BH induces autophagy in GSCs. (C) SQSTM1 and LC3 II protein levels analyzed in DMC-BH treated GSCs in the presence of the autophagy inhibitor, 3-MA (1 mM; 3h) by western blotting. (D) Cell viability analyzed by MTT assay. (E) Western analysis of cleaved caspase-3 analyzed in GSCs incubated with DMC-BH +/- 3-MA by western blotting. (F) Western analysis of LC3 II protein levels in GSCs incubated with DMC-BH (100 μM and 20 μM) and/or z-VAD-fmk for 24 h; n =3.