Research Paper Volume 12, Issue 14 pp 14775—14790
The circular RNA circEIF3M promotes breast cancer progression by promoting cyclin D1 expression
Figure 9. CCND1 is directly targeted by miR-33a. (A) Schematic illustration of CCND1 3’UTR-WT and CCND1 3’UTR-Mut luciferase reporter vectors. (B, C and E) Relative mRNA and protein levels of CCND1 were detected in cells after transfected with miR-NC, miR-33a, NC inhibitor and miR-33a inhibitor using qRT-PCR, respectively. (D) The relative luciferase activities were detected after transfected with CCND1 3’UTR-WT or CCND1 3’UTR-Mut and miR-33a mimics or miR-NC, respectively. (F and G) Relative expression of CCND1 was detected by qRT-PCR in cells transfected with indicated vectors, circEIF3M or sh-circEIF3M. Data were indicated as mean ± SD, **P < 0.01.