Research Paper Volume 12, Issue 14 pp 14808—14818

Hsa_circ_0044226 knockdown attenuates progression of pulmonary fibrosis by inhibiting CDC27

Figure 4. Hsa_circ_0044226 knockdown suppresses TGF-β1-induced EMT in RLE-6TN cells by downregulating CDC27. (A) qRT-PCR analysis of the levels of CDC27 expression in RLE-6TN cells overexpressing CDC27. (BH) RLE-6TN cells were pretreated with 10 ng/mL TGF-β1 for 48 h and then transformed with hsa_circ_0044226 shRNA2 and CDC27 for 72 h. Expression levels of E-cadherin, α-SMA, collagen III, fibronectin 1, CDC27 and p21 were detected with western blotting. Relative expression levels were quantified by normalization to β-actin. **P < 0.01 vs. Blank group. ##P < 0.01 vs. TGF-β1 group. ^P < 0.05, ^^P < 0.01 vs. TGF-β1 + shRNA2 group.