Figure 5. Hsa_circ_0044226 knockdown suppresses pulmonary fibrosis by inhibiting EMT. (A, B) RLE-6TN cells were pretreated with 10 ng/mL TGF-β1 for 48 h and then transformed with hsa_circ_0044226 shRNA2 and CDC27 for 72 h. EMT was assessed based on relative fluorescence of E-cadherin, α-SMA and DAPI staining determined using IHC. **P < 0.01 vs. Blank group. ##P < 0.01 vs. TGF-β1 group. ^P < 0.05, ^^P < 0.01 vs. TGF-β1 + shRNA2 group.