Figure 6. Hsa_circ_0044226 knockdown alleviates pulmonary fibrosis in vivo. (A) RT-qPCR analysis of the levels hsa_circ_0044226 expression in lung tissues from BLM-treated model mice. (B, C) Collagen deposition and lung fibrosis determined using H&E and Masson’s trichrome staining. Blue arrow, collagen fibers. (D) Total lung fibrotic area was measured using Image-Pro Plus. (E) IHC staining of ki67 in lung tissues from mice. (F) Expression level of p21 was detected with western blotting. Relative expression level was quantified by normalization to β-actin. **P < 0.01 vs. Blank group. ##P < 0.01 vs. BLM group.