Research Paper Volume 12, Issue 14 pp 14885—14896

Circular RNA DHX33 promotes malignant behavior in ccRCC by targeting miR-489-3p/MEK1 axis

Figure 4. circDHX33 targeted miR-489-3p as a miRNA sponge. (A) circDHX33 distribution was explored by subcellular fractionation assay. (B, C) Target miRNAs of circDHX33 was predicted by miRanda and RNAhybrid. (D, E) The relative levels of miRNA candidates in 786O and A498 cells lysates were detected by pull-down assay. (F) MiR-489-3p had the complementary sites with the circDHX33. (G) MiR-489-3p mimics reduced the luciferase activity of the circDHX33-WT group. (H) The correlation between circDHX33 and miR-489-3p was confirmed by RIP assay. *p<0.05, **p<0.01.