Research Paper Volume 12, Issue 14 pp 14885—14896

Circular RNA DHX33 promotes malignant behavior in ccRCC by targeting miR-489-3p/MEK1 axis

Figure 6. MEK1 served as the target of miR-489-3p. (A, B) MEK1 was a potential putative target gene of miR-489-3p. (C) MEK1 expression was significantly upregulated in KIRC tissues. (D, E) MEK1 expression in ccRCC tissues was determined by RT-qPCR and IHC. (F, G) High MEK1 expression was significantly associated with poor prognosis in patients. (H, I) MiR-489-3p mimics reduced MEK1 expression in 786O and A498 cells. (J) MiR-489-3p mimics reduced luciferase activity of the MEK1-Wt group. (K) miR-489-3p expression was negatively correlated with MEK1 expression in ccRCC tissues. KIRC: Kidney renal clear cell carcinoma. *p<0.05, **p<0.01.