Research Paper Volume 12, Issue 14 pp 14897—14917

Neuroprotective and neurogenic effects of novel tetramethylpyrazine derivative T-006 in Parkinson’s disease models through activating the MEF2-PGC1α and BDNF/CREB pathways

Figure 4. T-006 prevents the loss of SN DA neuron loss by activating MEF2D/PGC1α/GSK3β signal pathway. Representative images of middle brain sections co-stained with antibodies against (A, D) TH (green) and p-GSK3β (red); (B, E) TH (red) and MEF2D (green); (C, F) TH (green) and PGC1α (red). DAPI (blue) indicates nucleus. (G, H) Quantitative analysis of immunofluorescence intensity in TH-positive cells. Data are expressed as mean±SEM (n=3 to 4 per group). #P<0.05, ###P<0.001 vs. sham group, and *P<0.05, **P<0.01 vs. MPP+ or 6-OHDA group.