Research Paper Volume 12, Issue 14 pp 14897—14917

Neuroprotective and neurogenic effects of novel tetramethylpyrazine derivative T-006 in Parkinson’s disease models through activating the MEF2-PGC1α and BDNF/CREB pathways

Figure 5. T-006 protects against neurotoxin-induced neurotoxicity in neurons. CGNs or corrected DA neurons were pretreated with T-006 at the indicated concentration for 2 hours followed by 150 μM MPP+ or 30 μM 6-OHDA treatment for 24 hours. (A, I) MTT assays to evaluate the cell viability. (B, J) Measurements of the LDH release. (C) ROS production assessed by DCF fluorescence intensity. (D) Hoechst staining to show the apoptotic cells. (E) Statistical analysis of the number of pykonitic nuclei of (D). (F) Mitochondrial membrane potential tested by JC-1 kit. (G) Complex I activity evaluation. (H) Intracellular ATP content measurement. Results are representative of three independent experiments as the mean±SEM. ###P<0.001 vs. control (Ctrl) group, and*P<0.01, **P<0.01 and ***P<0.001 vs. MPP+ or 6-OHDA group.