Research Paper Volume 12, Issue 14 pp 14918—14932

RP11-295G20.2 facilitates hepatocellular carcinoma progression via the miR-6884-3p/CCNB1 pathway

Figure 5. CCNB1 was the target of miR-6884-3p. (A) Targetscan analysis result showed that miR-6884-3p had a binding site with CCNB1. (B) Dual-luciferase reporter gene assay was used to confirm the target relationship between miR-6884-3p and CCNB1 in Hep3B and MHCC-97H cells. RT-qPCR (C) and western blotting (D) were used to determine the expression of CCNB1 in HCC tissues and adjacent tissues; (E) The expression relationship between miR-6884-3p and CCNB1 was evaluated by Spearman’s correlation analysis. (F, G) The expression of CCNB1 was detected by RT-qPCR and western blotting in Hep3B and MHCC-97H cells,*p<0.05.