Research Paper Volume 12, Issue 14 pp 14918—14932

RP11-295G20.2 facilitates hepatocellular carcinoma progression via the miR-6884-3p/CCNB1 pathway

Figure 6. CCNB1 overexpression partially reverses the effects of miR-6884-3p on Hep3B and MHCC-97H cells. Hep3B and MHCC-97H cells transfected with the miR-6884-3p mimics with pcDNA3.1 or pc-CCNB1 were subjected to the following assays. (A) Western blotting was used to detect the protein level of CCNB1,*P < 0.05. (BF) The CCK-8 assay, colony formation assay, cell apoptosis assay, and Transwell migration and invasion assays were performed to test the proliferation, colony formation, apoptosis, migration, and invasion of Hep3B and MHCC-97H cells, respectively,*P < 0.05.