Research Paper Volume 12, Issue 14 pp 14918—14932

RP11-295G20.2 facilitates hepatocellular carcinoma progression via the miR-6884-3p/CCNB1 pathway


Figure 7. Inhibition of miR-6884-3p partially reverses the effects of RP11-295G20.2 silence on Hep3B and MHCC-97H cells. Hep3B and MHCC-97H cells transfected with the si-RP11-295G20.2 with NC inhibitor or miR-6884-3p inhibitor were subjected to the following assays. (A, B) RT-qPCR was used to detect the protein level of miR-6884-3p,*P < 0.05. (C) Western blotting was used to detect the protein level of CCNB1,*P < 0.05. (DH) The CCK-8 assay, colony formation assay, cell apoptosis assay, and transwell migration and invasion assays were performed to test the proliferation, colony formation, apoptosis, migration, and invasion of Hep3B and MHCC-97H cells, respectively,*P < 0.05.