Research Paper Volume 12, Issue 14 pp 14966—14977

MiR-155 contributes to intestinal barrier dysfunction in DSS-induced mice colitis via targeting HIF-1α/TFF-3 axis

Figure 4. MiR-155 antagomir ameliorated intestinal barrier dysfunction in DSS-induced colitis. (A) Transmission electron microscopic (TEM) assay of colon specimens (magnification ×5000). The damages of epithelial cell villous and epithelial cells were significant in DSS and ant-NC group. (B) Western blotting analysis of TJ proteins (ZO-1, occludin, and claudin-1). (CE) representative protein levels of ZO-1/β-actin, occludin/β-actin, and claudin-1/β-actin (n=3). (FH) Relative mRNA of ZO-1, occludin, and claudin-1 in mice colonic tissue were measured by qPCR (n=5). Each bar represents mean ± SD, #P > 0.05, *P < 0.05, **P < 0.01 vs. DSS group.