Research Paper Volume 12, Issue 14 pp 14966—14977

MiR-155 contributes to intestinal barrier dysfunction in DSS-induced mice colitis via targeting HIF-1α/TFF-3 axis

Figure 6. MiR-155 dilapidated intestinal barrier may by targeting HIF-1α/TFF-3 axis. (A) Wild-type sequences of HIF-1α for miR-155 target. (B) Dual luciferase report assay of HIF-1α 3’-UTR wild type or MUT along with miR-155. #P > 0.05, *P < 0.05 vs. Negative Control. (C) The hypoxic station in normal and DSS-induced colitis mice colon labeled by hypoxia Probes. Green was the positive signal. (magnification ×100). (D) Relative mRNA of HIF-1α in mice colonic tissue were measured by qPCR (n=5). (E) Western blotting analysis of HIF-1α proteins and TFF-3 proteins in mice colon. (F, G) Representative protein levels of TFF-3/β-actin and HIF-1α/β-actin(n=3). Each bar represents mean ± SD. #P > 0.05, *P < 0.05, **P < 0.01 vs. DSS group.