Figure 4. PTEN regulates HUVEC function. (A) PTEN expression was measured by qRT-PCR in the different treated groups. (B–C) Effect of diabetic exosomes on HUVEC proliferation measured by CCK8 and EDU incorporation assays. Scale bar: 100 μm. (D–E) The effect of PTEN on the levels of the proliferation-related proteins Cyclin D1 and Cyclin D3, assessed by WB and qRT-PCR analysis. (F) The effect of PTEN on the levels of the apoptosis-related proteins Bax and Bcl-2. (G–I) Effects of PTEN on tube formation ability of HUVECs. Scale bar: 200 μm. Data are the mean ± SD of three independent experiments. *P <0.05, **P <0.01, ***P <0.001.