Research Paper Volume 12, Issue 14 pp 14978—14989

Inhibition of miRNA-152-3p enhances diabetic wound repair via upregulation of PTEN

Figure 6. Inhibition of miR-152-3p enhanced HUVEC function. (A) The qRT-PCR result confirmed the effect of miR-152-3p agonist and antagonist on the expression of miR-152-3p. (B) CCK8 proliferation assay was performed, and the results demonstrated that proliferation of HUVECs was increased after miR-152-3p antagonist treatment. (CD) The proliferation-related mRNAs in the different groups were measured using qRT-PCR and WB analyses. (E) The apoptosis-related mRNAs were assessed using qRT-PCR analysis. (FH) Effects of miR-152-3p on the tube formation ability of HUVECs. Scale bar: 200 μm. Data are the mean ± SD of three independent experiments. *P <0.05, **P <0.01, ***P <0.001.