Research Paper Volume 12, Issue 14 pp 14990—15001

Lysine-specific histone demethylase 1B (LSD2/KDM1B) represses p53 expression to promote proliferation and inhibit apoptosis in colorectal cancer through LSD2-mediated H3K4me2 demethylation

Figure 6. LSD2 inhibits p53 expression via demethylation of H3K4me2 through binding to the p53 promoter. (A, B) Real-time PCR assays to detect p53 mRNA expression for vector, LSD2, ShNC, and Sh-LSD2 in HCT-116 and LoVo cells. (C, D) p53 and H3K4me2 protein expression measured by Western blot for vector, LSD2, and ShNC in HCT-116 and LoVo cells. (E, F) P53 promoter activity in vector, LSD2, ShNC and Sh-LSD2 in HCT-116 and LoVo cells measured by luciferase assay. Data were expressed as means ± SEM. (G) ChIP assay in LoVo cells showing the interaction between LSD2 and H3K4me2 at the p53 promoter. **p < 0.01.