Research Paper Volume 13, Issue 4 pp 4962—4975

Methylation-dependent MCM6 repression induced by LINC00472 inhibits triple-negative breast cancer metastasis by disturbing the MEK/ERK signaling pathway

TNBC tissues and cells exhibit low expression of LINC00472 and the expression of LINC00472 is negatively correlated with Ki-67 expression. (A) The expression of LINC00472 in normal and TNBC tissues in microarray dataset GSE61724, blue referring to normal tissues and red referring to TNBC tissues. (B) The expression of LINC00472 in normal and TNBC tissues normalized to GAPDH determined by RT-qPCR (n = 42). (C) The expression of LINC00472 in normal and TNBC cell lines normalized to GAPDH determined by RT-qPCR. (D) The correlation between LINC00472 and Ki-67 analyzed by Pearson’s correlation coefficient. * p vs. the normal tissue or the MCF-10A cell line. The results were measurement data and expressed as mean ± standard deviation. Data comparison in (B) was analyzed by paired t-test and in (C) was analyzed by one-way ANOVA, followed by Tukey’s post hoc test. The experiment was conducted 3 times independently.

Figure 1. TNBC tissues and cells exhibit low expression of LINC00472 and the expression of LINC00472 is negatively correlated with Ki-67 expression. (A) The expression of LINC00472 in normal and TNBC tissues in microarray dataset GSE61724, blue referring to normal tissues and red referring to TNBC tissues. (B) The expression of LINC00472 in normal and TNBC tissues normalized to GAPDH determined by RT-qPCR (n = 42). (C) The expression of LINC00472 in normal and TNBC cell lines normalized to GAPDH determined by RT-qPCR. (D) The correlation between LINC00472 and Ki-67 analyzed by Pearson’s correlation coefficient. * p < 0.05 vs. the normal tissue or the MCF-10A cell line. The results were measurement data and expressed as mean ± standard deviation. Data comparison in (B) was analyzed by paired t-test and in (C) was analyzed by one-way ANOVA, followed by Tukey’s post hoc test. The experiment was conducted 3 times independently.