Research Paper Volume 12, Issue 14 pp 15050—15057

Establishing a density-based method to separate proliferating and senescent cells from bone marrow stromal cells

Figure 1. Isolation and Identification of BMSCs by Phenotypic Characterization and Multipotent Differentiation Potential. (A) Cells were isolated from the femurs and tibias of 3- to 4-week-old mice shown at P0 and P3. Cells are attached at P3. Scale Bar=200μm. (B) Flow cytometric analysis of cell surface markers on isolated BMSCs indicates Scal-1+ CD29+ CD11b- CD45- CD105-. (C) Differentiation capacity of BMSCs: ALP staining of cells cultured in osteogenic induction medium for 7 days (upper-left image); alizarin red staining of cells cultured in osteogenic induction medium for 21 days (upper-right image); oil red O staining of cells cultured in adipogenic induction medium for 7 days (lower-left image); and alcian blue staining of cells cultured in chondrogenic induction medium for 14 days (lower-right image). P0, passage 0; P2, passage 2; P3, passage 3. Scale Bar=200μm.