Figure 5. Testosterone ameliorated vascular fibrosis in aging-related arterial stiffness. (A1-4) Representative H&E staining, Masson trichrome staining, Picrosirius red staining and Verhoeff Van Gieson (scale bar: 20μm). (B–E) Quantitative analyses of collagen content, elastin content, collagen content/elastin content and average aortic wall architecture score. (F) Immunohistochemical staining for collagen I and III (brown staining considered positive staining; scale bar: 20μm). (G) Representative Western blots of collagen I and III. (H) Western blot analyses of collagen I and III. Data are mean ± SD; *P < 0.05 and **P < 0.01 vs. WT young group; †P < 0.05 and ††P < 0.01 vs. WT old group; &P < 0.05, &&P < 0.01 and &&&P < 0.001 vs. Axl-/- young group; #P<0.05 vs. Axl-/- old group; A-F: n = 5 for each group, G-H: n = 3 for each group.