Organ-specific insulin sensitivity by activation of AKT/S6 in GHRH-/- mice. (A–C) Activation of AKT and S6 in kidney. (D–F) Activation of AKT and S6 in spleen. (G–I) Activation of AKT and S6 in lung. (J–L) Activation of AKT and S6 in heart. The mice were fasted for 4 hours before i.p. injection with porcine insulin (1 IU/kg of body weight). Tissues were collected 20 min after following insulin i.p. injection. All data (means ± sem) were expressed as fold change compared to vehicle treated WT controls (defined as 1.0) (n=4 for WT group; n=6 for GHRH-/- mice). Statistical analysis was performed by unpaired Student’s t-test, * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001.