Research Paper Volume 12, Issue 18 pp 18033—18051

Insulin sensitivity in long-lived growth hormone-releasing hormone knockout mice

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Figure 6.

Tissue-specific phosphorylation of ERK1/2 by insulin stimulation in GHRH-/- mice. (A, B) Activation of ERK1/2 in kidney. (C, D) Activation of ERK1/2 in spleen. (E, F) Activation of ERK1/2 in heart. (G, H) Activation of ERK1/2 in lung. The 4 hours fasted mice were injected i.p. with porcine insulin (1 IU/kg of body weight). After 20 min, tissues were collected to perform western blots. All data (means ± sem) were expressed as fold change compared to vehicle treated WT controls (defined as 1.0) (n=4 for WT group; n=6 for GHRH-/- mice). Statistical analysis was performed by unpaired Student’s t-test, ** P < 0.01, *** P < 0.001 and **** P < 0.0001.