Research Paper Volume 12, Issue 14 pp 15121—15133

Megakaryocytes promote osteoclastogenesis in aging


Figure 2. Aging increases bone marrow MK number. (A) Flow cytometry was used to determine the total number of CD45+CD41+ MKs in the bone marrow of young and aged C57BL/6J female and male mice. The data are presented as mean ± SD of four independent experiments with *p<0.05 (N=4/group). (B) Bone marrow CD45+CD41+ MKs were isolated by FACS from young and aged mice (males and females were combined) and used for real-time PCR analysis of relative Mpl mRNA expression between groups. The Ct cycle range was 28.99–33.83. The data are presented as mean ± SD of three independent experiment (****p<0.0001; N=3-6/group).