Research Paper Volume 12, Issue 17 pp 16921—16935

Epigenetic upregulation of HOXC10 in non-small lung cancer cells

Figure 2. Promotion of cell growth by overexpression of HOXC10 in A549 cells. (A) A549 cells that stabily overexpress HOXC10 (pCDH_HOXC10) or control (pCDH_GFP) were generated as described in Methods. Total RNA was extracted and RNA levels of HOXC10 were assessed using qRT-PCR. A fold change was obtained by normalizing to the house keeping gene GAPDH and setting the values from the negative control pCDH_GFP to one. (B) Similar to part A except that the protein levels of HOXC10 were assessed using immnoblotting. (C) Similar to part A except that cell viability was measured using MTT assays. A fold change of the MTT values was obtained by setting the values from the negative control pCDH_GFP to one. (D) Similar to part A except that apoptosis was measured using Annexin V-FITC. Percentage of apoptotic cells were compared among the groups. (E) The expression of apoptosis related proteins in A549 cells with pCDH_HOXC10 or control transfected was measured by western blot analysis. When presented, means and standard deviations were obtained from at least 3 independent experiments. *, **, and *** indicate a P value < 0.05, 0.01, and 0.001, respectively.