Research Paper Volume 12, Issue 17 pp 16921—16935

Epigenetic upregulation of HOXC10 in non-small lung cancer cells

Figure 3. Inhibition of cell growth by knockdown of HOXC10 in NCI-H23 cells. (A) The HOXC10-specific siRNAs (siRNA 1-3) or negative control siRNA (NC) were transfected into NCI-H23 cells. Total RNA was extracted and RNA levels of HOXC10 were assessed using qRT-PCR. A fold change was obtained by normalizing to the house keeping gene GAPDH and setting the values from the negative control siRNA (NC) transfected group to one. (B) Similar to part A except that the protein levels of HOXC10 were assessed using immnoblotting. (C) Similar to part A except that cell viability was measured using MTT assays. A fold change of the MTT values was obtained by setting the values from the negative control siRNA (NC) transfected group one. (D) Similar to part A except that apoptosis was measured using Annexin V-FITC. Percentage of apoptotic cells were compared among the groups. (E) The expression of apoptosis related proteins in NCI-H23 cells with HOXC10-specific siRNA3 or negative control siRNA transfected was measured by western blot analysis. When presented, means and standard deviations were obtained from at least 3 independent experiments. *** indicates a P value < 0.001.