Research Paper Volume 12, Issue 17 pp 16921—16935

Epigenetic upregulation of HOXC10 in non-small lung cancer cells

Figure 7. G-quadruplex-mediated regulation of HOXC10 expression. (A) NCI-H23 cells were exposed to the G4 disruptive ligand TAP1 (2 μM) for 4 days. Total RNA was extracted and assessed for the RNA levels of HOXC10 using qRT-PCR. A fold change was obtained by normalizing to the housekeeping gene RPLP0 and setting the values from the DMSO control group to one. (B) Similar to part D except that the BG4 ChIP signals were compared between the DMSO control and TAP1 treated groups. (C) A549 cells were exposed to the G4 enhancing ligand pyridostatin (PYRI) (5 μM) for 48 hrs. Total RNA was extracted and assessed for the RNA levels of HOXC10 using qRT-PCR. A fold change was obtained by normalizing to the housekeeping gene RPLP0 and setting the values from the DMSO group to one. When presented, means and standard deviations were obtained from at least 3 independent experiments. * and ** indicate a P value < 0.05 and 0.01, respectively.