Research Paper Volume 12, Issue 14 pp 15134—15156

Signatures of cell stress and altered bioenergetics in skin fibroblasts from patients with multiple sclerosis

Figure 5. Lactate production is increased in MS skin fibroblasts. Skin fibroblasts from MS (n = 25-29), ALS (n = 9-10), and control cells (n = 20-21) were incubated in media (without FBS) for 24 hours or 48 hours prior to measuring lactate concentrations in the media. (A) Significant changes between groups were determined using two-way ANOVA post hoc Tukey test (repeated measures with mixed model). Each data point represents a unique skin fibroblast sample. Determined lactate concentrations were normalized to cellular protein content. The average of replicates is shown with the 95% confidence interval. (B) Summary data of detected lactate concentrations after 24 or 48 hours. The average of all data is shown per group with the 95% confidence interval. *, P < 0.05, ***, P < 0.001; ****, P < 0.0001. Abbreviations: ALS, amyotrophic lateral sclerosis; Ctrl, control; MS, multiple sclerosis.