Research Paper Volume 13, Issue 10 pp 14469—14481

Silencing lncRNA XIST exhibits antiproliferative and proapoptotic effects on gastric cancer cells by up-regulating microRNA-132 and down-regulating PXN

Inhibition of LncRNA XIST restrains tumor formation ability, cell proliferation and migration and enhances apoptosis in gastric cancer. The SGC7901 cells were transfected with siRNAs targeting XIST, namely, si-XIST-1 and si-XIST-2. (A) The expression of lncRNA XIST determined by RT-qPCR; (B) Cell proliferation ability assessed by EdU assay (× 400); (C) Cell migration ability evaluated by scratch test (× 40); (D) Cell apoptosis ability evaluated by flow cytometry; (E) The effect of lncRNA XIST on tumor formation in nude mice injected with the stably transfected SGC7901 cells. All measurement data and statistical results were expressed as mean ± standard deviation; *, p

Figure 2. Inhibition of LncRNA XIST restrains tumor formation ability, cell proliferation and migration and enhances apoptosis in gastric cancer. The SGC7901 cells were transfected with siRNAs targeting XIST, namely, si-XIST-1 and si-XIST-2. (A) The expression of lncRNA XIST determined by RT-qPCR; (B) Cell proliferation ability assessed by EdU assay (× 400); (C) Cell migration ability evaluated by scratch test (× 40); (D) Cell apoptosis ability evaluated by flow cytometry; (E) The effect of lncRNA XIST on tumor formation in nude mice injected with the stably transfected SGC7901 cells. All measurement data and statistical results were expressed as mean ± standard deviation; *, p < 0.05 compared with cells transfected with si-NC or the mice injected with si-NC-transfected cells; comparisons were conducted using one-way ANOVA. The experiment was repeated 3 times.