Figure 4. LncRNA XIST competitively binds to miR-132 and modulates PXN. (A) Binding region between miR-132 and PXN; (B) Relative luciferase activity between miR-132 and PXN; (C) Protein bands of PXN measured by western blot assay; (D) PXN protein expression in the SGC7901 cells transfected with miR-132 mimic or inhibitor or co-transfected with miR-132 inhibitor and si-XIST. All measurement data and statistical results were expressed as mean ± standard deviation; *, p < 0.05 compared with the NC group. Comparisons between two groups were analyzed by t-test, while comparison among multiple groups were analyzed using one-way ANOVA. The experiment was repeated 3 times.